And purified utilizing this protocol was lately used successfully for RNA-Seq, and RNA from cone scales in the cycad Macrozamia lucida For grinding challenging tissue or ligneous tissue, including cone scales from gymnosperms, the tissue was floor below liquid nitrogen inside a mortar and pestle which was sterilized and baked https://rnabeads05824.bloggerswise.com/36319091/rapid-isolation-of-plasmid-dna-an-overview